American society for mass spectrometry asms, and has served many different positions. Before we talk about interpreting spectra, lets discuss how they are generated in the first place. Energy is redistributed and finally low mass products are formed. Decomposition of electron ionization mass spectra for space. Introduction to mass spectrometry syllabus for chem 395455. It is concise, wellwritten, and very comprehensible. International association of protein structure analysis and proteomics. Turecek published by university science books, mill valley, 1993 isbn.
Proteomicsprotein identification mass spectrometryreferences. For simple lcms data handling the vendor specific software is usually sufficient. Mclafferty s text has become a classic for classroom or self study concerned with interpreting mass spectra in order to discern molecular structures or identities of compounds. Mclaffertys most popular book is interpretation of mass spectra. Fred mclafferty was born in evanston, illinois in 1923, but attended grade school in omaha, nebraska, graduating from omaha north high school in 1940. If, for a carboncontaining compound, peak a has intensity 100, then higher mass peaks have intensity.
Structure elucidation of small molecules fiehn lab. Flock for their efforts and to the staff of the local libraries for their friendly support. Previous chapter recommend to your librarian download as pdf export citation next chapter. Mass spectrometry mclafferty rearrangement problems asked. Mass spectrometry mclafferty rearrangement problems. It is commonly used for the identification of organic compounds from electron ionization mass. A few compounds have mass spectra which dont contain a molecular ion peak, because all the molecular ions break into fragments. Common fragmentation processes for organic molecules are the mclafferty rearrangement and alpha cleavage. The leading scientist in this field was fred mclafferty who together with frantisek turecek has written a very famous text book. These metrics are regularly updated to reflect usage leading up to the last few days.
Ms interpretation i identification of the molecular ion. October 1981 international journal of mass spectrometry and ion physics harry j. In mass spectrometry, one generates ions from a sample to be analyzed. Interpretation of mass spectra, say the authors, aims at correlating ion dissociation mechanisms on a much broader scale, with emphasis on basic attributes such as ionization energies, proton affinities, and bond dissociation energies. Not all mass spectra of a given substance are the same. He is also known for electron capture dissociation, a method of fragmenting gas phase ions. Mass spectrometry mass spectrometry is the most accurate method for determining the molecular mass of the compound and its elemental composition. Westinghousenew method for accurate gas analysisa mass spectrometer 1943 1. It delivers practical and sustainable innovations to enable significant advancements in areas such as healthcare delivery, life sciences, environmental management, energy, food safety, and water quality. Mass spectrum interpretation fragmentation reactions 1. Mass spectrometry in chemical research and production, appl. Article views are the countercompliant sum of full text article downloads since november 2008 both pdf.
Caprioli, vanderbilt university, nashville fast atom bombardment. The first and basic step in any interpretation of mass spectra is always the determination of molecular weight, which. Veith, technical university of darmstadt field desorption, r. It is also called as positive ion spectra or line spectra. If you are starting to interpret ei mass spectra, this is the perfect guide to start with. In order to interpret the mass spectrum, one should attain an understanding of the ionisation process. The mclafferty rearrangement is an organic reaction seen in mass spectrometry. When only a mass spectrum of the sample is available, the results tend to be. He is best known for the mclafferty rearrangement reaction that was observed with mass spectrometry. Introduction to mass spectrometry syllabus for chem 395. Turecek published by university science books, mill valley, 1993 isbn 0. Published by university science books 1993 isbn 10. Fragmentation of organic ions and interpretation of ei mass. Jimenez, department of chemistry and biochemistry ucb 215, cooperative institute for research in the environmental sciences.
May 23, 2018 in this video we will try to understand basics of mass spectrometry. Fred warren mclafferty is an american chemist known for his work in mass spectrometry. Mclafferty s most popular book is interpretation of mass spectra. Collisioninduced dissociation pathways of anabolic. Digital notes and study tools builtin study tools include highlights, study guides, annotations, definitions, flashcards, and. Interpretation of mass spectra a standard handbook of mass spectrometry by mclafferty turecek lcms for simple lcms data handling the vendor specific software is usually sufficient.
Frantisek tureek and a great selection of related books, art and collectibles available now at. The molecule breaks apart in consistent ways that chemists can predict. For structure elucidation and mass spectral identification highchem mass frontier and acdms manager are required. Most interpretation techniques are applicable to spectra. Frantisek tureek and a great selection of related books. Interpretation of mass spectra, 4th edition, by fred mclafferty, published by university science books, 0935702253 interpretation of mass spectra, 4th ed. Article views are the countercompliant sum of full text article downloads since november 2008 both pdf and html across all institutions and individuals. Using ei mass spectra of small molecules from the nist database, we demonstrate that it is possible to determine atomic ratios for oc, hc, and nc, as well as the ratio for total organic mass to organic carbon mass omoc. In certain cases, adduct ions may dominate the spectra. In this video we will try to understand basics of mass spectrometry. Alternative to mclafferty, somewhat easier to read lee, a beginners guide to mass. Mass spectrometry allows us to measure the masses of atoms and molecules, and also obtain information about their chemical structure. Sauerwein to use a large set of electron ionization mass spectra from the nistepanih mass spectral library is also gratefully acknowledged. Interpretation of mass spectra by mclafferty, fred w.
If good inferences could be made, use the remainder of the mass spectrum to try to elucidate structural features or isomerism. One part will concentrate on the interpretation of mass spectra. Most times, a carboncarbon bond breaks and the atoms do. The specific objectives of this course are to acquaint the student with basic aspects of mass spectrometry. Interpretation of mass spectra, say the authors, aims at correlating ion dissociation mechanisms on a much broader scale, with emphasis on basic attributes such as ionization energies, proton read more. The ionized molecule often fragments into smaller ionsradicals. Interpretation of mass spectra by mclafferty fred w turecek. Elemental analysis of organic species with electron. For a large enough number of carbons, m is no longer most intense peak.
Interpretation of mass spectra of organic molecules. Mass spectrometry is the key enabling technology for the 21st century. However, there are characteristic ion series at lo wer mass that also give insights into molecular structure. That isnt a problem you are likely to meet at alevel.
Proteomicsprotein identification mass spectrometry. Turecek, interpretation of mass spectra, university science. This is exemplified by the esimsms behavior of naled where the peak at m z 127 obeys this rule. Lecture notes in eimass spectrometry by torben lund. Fragmentation of organic ions and interpretation of ei. Mass spectroscopy minnesota state university moorhead. The molecular weight of a pure compound from the identification of the parent peak. Alternative to mclafferty, somewhat easier to read lee, a beginners guide to mass spectral interpretation, 1998. It is a histogram usually acquired using an instrument called a mass spectrometer. Everyday low prices and free delivery on eligible orders. For example, in the mass spectrum of pentane, the heaviest ion has an mz value of 72. Hence, the mass spectrum of a sample is a pattern representing the distribution of ions by mass more correctly. Interpretation of mass spectra a standard handbook of mass spectrometry by mclafferty turecek.
Electron capture dissociation for structural characterization of multiply charged protein cations, anal. First, the fundamental notions of mass spectrometry are explained, so that the reader can easily cover this chapter graphs, main pick, molecular ion, illogical pick, nitrogen rule, etc. Sysoev a, adamov a, viidanoja j, ketola ra, kostiainen r, kotiaho t. Most mass spectra are not trivial to decipher due to. With roland gohlke, he pioneered the technique of gas chromatography mass spectrometry. Citations are the number of other articles citing this article, calculated by crossref and updated daily. Of course, a single chapter cannot be comprehensive and thus, further reading may be desirable.
Read your book anywhere, on any device, through redshelfs cloud based ereader. The first proton originates from the system and the second from proton transfer. Chromatographic and mass spectral studies on methoxy methyl. Isotopic percentage and nominal mass calculation are also. Buy interpretation of mass spectra 1993 by mclafferty, fred w.
Wesener, bayer industry services, leverkusen chemical ionization, j. Mass spectrometry and interpreting mass spectra compound. Results in complex mass spectra with fragment ions, useful for. Information is concentrated at higher mass in the primary fragmentations neutral losses. Extensively revised and updated, this new edition, as in pr. A mass spectrum can represent many different types of information based on the type of mass spectrometer and the specific experiment applied. Interpretation of mass spectra third edition fred w. Metzger, university of oldenburg electron ionization and fragmentation of organic ions and interpretation of ei mass spectra, j. It is commonly used for the identification of organic compounds from electron ionization mass spectrometry. A mass spectrometer breaks apart the molecule being studied. My thanks are extended to the staff of my facility n. Additional analysis if no inferences are realistic from tables 3 and 4, attempt deeper analysis of the mass spectrum.
Interpretation of mass spectra, fw mclafferty and f. Graham cooks, hao chen, zheng ouyang, andy tao, yu xia and lingjun li. Mass spectroscopy mass spectroscopy is a technique causing the formation of the gaseous ions with or without fragmentation. Interpretation of production spectra of anabolic steroids and exploration of their collisioninduced dissociation cid pathways are of importance to mass spectrometric characterization of new designer steroids, and helpful in understanding specificity of product ions of anabolic steroids for their detection, identification, and. Mass spectra is a plot of relative abundance against mass tocharge ratio. Mclafferty rearrangement simple english wikipedia, the. Mclafferty has 16 books on goodreads with 27 ratings. Mass spectral interpretation is the method employed to identify the chemical formula, characteristic fragment patterns and possible fragment ions from the mass spectra. The subsequent interpretation of full scan and tandem mass spectra is more complicated due to a high number of possible functional groups. The urgent requirements of world war ii accelerated his undergraduate studies at the university of nebraska. Turecek and a great selection of related books, art and collectibles available now at. The following chapter introduces one of the key disciplines of organic mass spectrometry.
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